Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. PDF] Evidence that Increased Acetaminophen use in Genetically Vulnerable Children Appears to be a Major Cause of the Epidemics of Autism, Attention Deficit with Hyperactivity, and Asthma Semantic Scholar Full article: The development and hepatotoxicity of acetaminophen: reviewing over a century of progress Glutathione: Master Antioxidant, Reducing Oxidative Stress, and Detoxification